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Methylene Blue is a cationic phenothiazinium dye commonly called methylthioninium chloride. In aqueous solution it produces an intense blue colour and participates in reversible oxidation-reduction chemistry. Many commercial dye preparations contain crystal water, so the exact molecular formula, registry number and mass must be based on the supplied form. This product is listed for laboratory procurement in the pack size shown in its product name; pack size does not alter chemical identity. In educational and institutional settings, this material can support selected experiments, analytical method development or teaching exercises when matched to the procedure and suitable laboratory facilities. Correct reagent identification, applicable safety information and an appropriate method are essential before use. Physical presentation and grade alone do not establish fitness for all analytical or biological methods. Where the product name omits a hydrate form or formulation detail, verify that identity before calculating reagent masses or preparing quantitative solutions. All work should follow institutionally approved laboratory procedures and the specific product safety data sheet.
Product Specifications
| Specification | Details |
|---|---|
| Appearance | Dark green crystalline powder |
| Assay | 99% |
| Chemical Name | Methylene blue (hydrate form unspecified) |
| Alternate Names | Basic Blue 9; methylthioninium chloride; C.I. 52015 |
| Molecular Formula | Not confirmed; anhydrous C16H18ClN3S and hydrated forms differ |
| Empirical Formula | Not confirmed without hydrate identification |
| CAS Number | Not confirmed without hydrate identification |
| Molecular Weight | Not confirmed without hydrate identification |
| Grade | LR Laboratory Reagent |
Key Features of Methylene Blue
- Cationic phenothiazinium chromophore gives a deep-blue solution.
- Commonly used to colour biological samples in microscopy.
- Exhibits reversible redox chemistry under suitable conditions.
- Supplied as crystalline or powdered material, including hydrated forms.
- Useful for absorption and dye-binding studies with controlled concentration.
Laboratory Applications of Methylene Blue
- Staining of suitable biological smears for microscopy under validated protocols.
- Microscopy teaching on contrast and cellular morphology.
- Laboratory demonstrations of reversible dye redox reactions.
- Preparation of controlled stain solutions after form verification.
- Visible absorption measurements and adsorption research.
Why Choose Lab Wale to Buy Methylene Blue
Lab Wale lists Methylene Blue for institutional buyers sourcing laboratory chemicals and scientific supplies for teaching, research and analytical work. Clearly identifying the chemical and intended use helps laboratories select appropriate reagents and manage procurement documentation. Review the stated LR Laboratory Reagent grade together with the exact product identity, especially where hydrate or formulation details are not specified. Consult available product-specific safety and technical information, and confirm that the selected material meets the requirements of your laboratory method before purchasing.
Frequently Asked Questions About Methylene Blue
What is Methylene Blue used for in laboratories?
Methylene Blue is used in selected institutional laboratory settings. Examples include staining of suitable biological smears for microscopy under validated protocols. and microscopy teaching on contrast and cellular morphology. Procedures must be matched to the chemical form, equipment and safety restrictions. It should not be assumed suitable for unrestricted educational use.
What are the formula and CAS number of Methylene Blue?
The molecular formula is Not confirmed; anhydrous C16H18ClN3S and hydrated forms differ, and the CAS number is Not confirmed without hydrate identification. Some commercial products may have different hydrate forms or formulations. Confirm the manufacturer identification before making quantitative solutions or entering a substance into an institutional chemical inventory.
What key laboratory properties does Methylene Blue have?
In aqueous solution it produces an intense blue colour and participates in reversible oxidation-reduction chemistry. Many commercial dye preparations contain crystal water, so the exact molecular formula, registry number and mass must be based on the supplied form. These characteristics can inform experimental planning but do not replace method-specific validation or evaluation of reagent grade and safety.
Is Methylene Blue appropriate for school and research laboratory work?
Use depends on the application, chemical hazards and available engineering controls. Some studies are suitable for supervised educational settings, whereas hazardous mercury or nickel substances require specialist facilities and authorized personnel. Always review the SDS and institutional safety permissions before work.
How should Methylene Blue be stored and handled?
Store tightly closed and protected from moisture and strong light in a clean, dry chemical storage area. Prevent contamination of the dye and keep powder away from incompatible oxidizing agents as directed by the SDS. Label prepared staining solutions with concentration and preparation date. Verify hydrate form before quantitative formulation. Follow the manufacturer SDS for protective equipment, spill response,.
Storage Conditions for Methylene Blue
Store tightly closed and protected from moisture and strong light in a clean, dry chemical storage area. Prevent contamination of the dye and keep powder away from incompatible oxidizing agents as directed by the SDS. Label prepared staining solutions with concentration and preparation date. Verify hydrate form before quantitative formulation.
Precautions for Handling Methylene Blue
Read the applicable SDS and handle dye powders with gloves, eye protection and a coat. Avoid inhaling dust and prevent skin or eye contact; methylene blue can strongly stain surfaces and clothing. Prepare microscopy reagents with appropriate ventilation and clean up spills without dispersing dry particles. Dispose of concentrated dye solutions under laboratory waste procedures.


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