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Haematoxylin Stain Powder contains haematoxylin, a natural organic dye obtained from logwood and widely used in histological and microscopic staining methods. In routine histology, haematoxylin is commonly oxidised to haematin and used with a suitable mordant to produce staining of nuclei and other tissue structures. Its staining performance depends on the formulation, oxidation state, mordant, pH, and protocol; the powder alone should not be assumed to be a ready-to-use stain. Haematoxylin is represented by the molecular formula C16H14O6 and has a molar mass of approximately 302.28 g/mol. It is used in educational and research laboratories following an established method and appropriate chemical-handling precautions.
Product Specifications
| Specification | Details |
|---|---|
| Appearance | White to yellowish crystals or powder |
| Assay | 99% |
| Chemical Name | Haematoxylin |
| Alternate Names | Hematoxylin; hydroxybrazilin; hydroxybrasilin |
| Molecular Formula | C16H14O6 |
| Empirical Formula | C8H7O3 |
| CAS Number | 517-28-2 |
| Molecular Weight | 302.28 g/mol |
| Grade | LR Laboratory Reagent |
Key Features of Haematoxylin Stain Powder
- Organic dye associated with logwood and used in microscopy staining.
- Molecular formula C16H14O6 and molar mass approximately 302.28 g/mol.
- Used in histological staining systems to support visualisation of nuclei and tissue structures.
- Often requires oxidation and a suitable mordant as part of a defined staining formulation.
- Staining outcome depends on reagent formulation, pH, mordant, and procedure.
Laboratory Applications of Haematoxylin Stain Powder
- Histology teaching practicals demonstrating staining of nuclei in prepared tissue sections.
- Preparation of haematoxylin-based staining solutions according to an established laboratory method.
- Microscopy investigations requiring a haematoxylin component in a validated staining protocol.
- Research sample preparation for histological examination, where the formulation is appropriate for the specimen.
- Comparative staining studies examining the effect of mordant, pH, and protocol on tissue contrast.
Why Choose Lab Wale to Buy Haematoxylin Stain Powder
Lab Wale supports laboratory purchasing for schools, colleges, universities, research centres, and laboratory professionals by providing a convenient source for laboratory chemicals and scientific supplies. Before purchasing Haematoxylin Stain Powder, check the product identity, intended use, stated grade, and available technical or safety information to ensure the material matches the procedure. Selecting the correct chemical form and following the relevant laboratory method are important for reliable and appropriate use.
Frequently Asked Questions About Haematoxylin Stain Powder
What is haematoxylin stain powder used for?
Haematoxylin is used to prepare staining reagents for microscopy, especially histological methods that visualise nuclei and tissue structures. The powder generally requires formulation according to a defined method and is not automatically a ready-to-use stain.
What is the molecular formula and molecular weight of haematoxylin?
Haematoxylin has the molecular formula C16H14O6 and a molar mass of approximately 302.28 g/mol. These values refer to haematoxylin itself, not to a prepared staining solution containing other ingredients.
What is the CAS number of haematoxylin?
The commonly cited CAS Registry Number for haematoxylin is 517-28-2. Verify that the material supplied is haematoxylin rather than a formulated haematoxylin stain or a related compound.
Why are a mordant and oxidation step used in haematoxylin staining?
Many established histological methods convert haematoxylin to haematin and use a mordant to form a coloured complex that binds to tissue structures. The exact chemistry and procedure depend on the staining formulation and method.
How should haematoxylin powder be stored and handled?
Protect the material from light, moisture, and contamination, and keep the container tightly closed. Avoid dust and direct contact, wear suitable PPE, and follow the current SDS for storage, handling, and waste-disposal requirements.
Storage Conditions for Haematoxylin Stain Powder
Store in a tightly closed, labelled container in a cool, dry place, protected from excessive light and moisture. Avoid contamination and follow the supplier’s SDS for product-specific temperature and segregation requirements. Keep the container closed when not in use.
Precautions for Handling Haematoxylin Stain Powder
Review the current SDS before use. Avoid generating or inhaling dust and prevent contact with skin and eyes; use suitable gloves, protective clothing, and eye protection. Prepare staining solutions only by an established procedure, provide appropriate ventilation, and manage spills and waste under institutional rules.


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