Crystal Voilet Powder- 100gm Packs

Crystal Violet Powder, also known as Crystal Violet, Gentian Violet, or Basic Violet 3, is a synthetic cationic triphenylmethane dye widely used in microbiology and laboratory staining procedures. The anhydrous chloride salt has the chemical formula C₂₅H₃₀ClN₃ and a molecular weight of approximately 407.98 g/mol. It is characterised by its intense violet colour and strong staining properties. Crystal Violet is particularly important in Gram staining, where it acts as the primary stain for differentiating bacterial groups during microscopic examination. It is also used in selected microscopy, histological staining, and research procedures involving dye binding and colourimetric analysis. Its solubility in suitable solvents allows preparation of staining solutions according to established laboratory protocols. This 100 gram pack is intended for educational, analytical, and research laboratory applications where the specified reagent grade meets the method requirements. Appropriate protective equipment and safe chemical-handling practices are essential.

Product Specifications

Specification	Details
Appearance	Dark green to violet crystalline powder
Assay	99%
Chemical Name	Crystal Violet
Alternate Names	Gentian Violet, Basic Violet 3, Methylrosanilinium Chloride, C.I. 42555
Molecular Formula	C₂₅H₃₀ClN₃
Empirical Formula	C₂₅H₃₀ClN₃
CAS Number	548-62-9
Molecular Weight	407.98 g/mol
Grade	LR Laboratory Reagent

Key Features of Crystal Violet Powder

• Distinctive violet dye: Crystal Violet is a cationic triphenylmethane dye with strong violet coloration and useful staining characteristics.
• Defined chemical composition: The anhydrous chloride salt has the formula C₂₅H₃₀ClN₃ and a molecular weight of approximately 407.98 g/mol.
• Suitable for staining solutions: Its solubility in water and other appropriate solvents supports the preparation of laboratory staining formulations.
• Microbiology applications: Functions as the primary stain in the Gram staining procedure used for bacterial differentiation.
• Research relevance: Used in selected microscopy, specimen staining, dye-binding investigations, and colourimetric laboratory procedures.

Laboratory Applications of Crystal Violet Powder

• Gram staining: Used as the primary stain in Gram staining protocols for differentiating Gram-positive and Gram-negative bacteria when the complete validated procedure is followed.
• Educational microbiology practicals: Supports supervised laboratory exercises involving bacterial staining, microscopic observation, and microbial morphology.
• Microscopy and specimen staining: Used in selected staining protocols for laboratory specimens, depending on the sample type and method requirements.
• Dye-binding investigations: Used in research examining the interaction of dyes with biological materials, surfaces, and other suitable laboratory samples.
• Colourimetric analysis: Can be used in selected analytical procedures involving dye concentration and absorbance measurements when a validated method is available.

Why Choose Lab Wale to Buy Crystal Violet Powder

Lab Wale provides a convenient sourcing option for laboratory chemicals and scientific supplies used by schools, colleges, universities, microbiology laboratories, research centres, and laboratory professionals. When purchasing Crystal Violet Powder, check the chemical identity, stated grade, pack size, and available technical and safety information to ensure suitability for the intended procedure. The 100 gram pack provides a defined quantity for laboratory requirements. Selecting the appropriate reagent and following validated staining protocols supports consistent laboratory practice and responsible chemical handling.

Frequently Asked Questions About Crystal Violet Powder

What is Crystal Violet Powder used for in laboratories?

Crystal Violet is primarily used as a laboratory stain, particularly as the primary dye in Gram staining for bacterial differentiation. It is also used in selected microscopy, specimen staining, and dye-binding research procedures. The appropriate formulation and concentration depend on the validated protocol and intended application.

What is the molecular formula of Crystal Violet?

The molecular formula of anhydrous Crystal Violet chloride is C₂₅H₃₀ClN₃, with a molecular weight of approximately 407.98 g/mol. Commercial formulations may differ in composition or hydration state, so the supplied form should be confirmed before calculating reagent quantities or preparing laboratory solutions.

What is the CAS number of Crystal Violet Powder?

The CAS Registry Number for Crystal Violet chloride is 548-62-9. The compound is also known as Gentian Violet and Basic Violet 3. Confirming the chemical identity helps distinguish the intended dye from other violet-coloured laboratory stains and related triphenylmethane compounds.

Why is Crystal Violet used in Gram staining?

Crystal Violet serves as the primary stain in Gram staining. After iodine is applied, a dye complex forms within bacterial cells. Gram-positive bacteria generally retain this complex during decolourisation, while Gram-negative bacteria lose the primary stain more readily and are subsequently visualised using a counterstain.

How should Crystal Violet Powder be stored and handled safely?

Store the powder in a tightly closed, compatible container in a cool, dry location, protected from unnecessary light exposure. Avoid inhaling dust and prevent contact with the eyes and skin. Wear appropriate protective equipment, consult the applicable Safety Data Sheet, minimise exposure, and dispose of chemical waste through approved procedures.

Storage Conditions for Crystal Violet Powder

Store Crystal Violet Powder in a tightly closed, compatible container in a cool, dry, well-ventilated area, protected from excessive heat and unnecessary light exposure. Avoid contamination and airborne dust. Keep the container clearly labelled and segregate the material from incompatible substances according to the applicable Safety Data Sheet. Follow institutional chemical-storage requirements and supplier-specific instructions.

Precautions for Handling Crystal Violet Powder

Review the current Safety Data Sheet before use and wear suitable chemical-resistant gloves, safety goggles, and laboratory clothing. Avoid inhaling dust and prevent contact with the eyes and skin. Use appropriate ventilation when weighing the powder or preparing staining solutions. Crystal Violet has significant health-hazard concerns, including suspected carcinogenicity in relevant safety classifications, so minimise exposure and follow institutional controls. Prevent environmental release and dispose of solutions, contaminated materials, and residues through approved chemical-waste procedures.