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Acrylamide is an organic compound belonging to the unsaturated amide family, containing a reactive carbon–carbon double bond and an amide functional group. Its molecular formula is C3H5NO, and its molecular weight is approximately 71.08 g/mol. Acrylamide is a white crystalline solid that is readily soluble in water and can undergo polymerization to form polyacrylamide. It is an important reagent in molecular biology and biochemical research, particularly for preparing polyacrylamide gels used in electrophoresis and protein separation. It is also relevant to polymer chemistry investigations and studies of polymerization reactions. This 250 g laboratory pack is intended for appropriate research and synthesis applications where the stated reagent specifications meet the experimental requirements. Acrylamide monomer is highly hazardous, can be absorbed through the skin, and presents neurotoxicity and carcinogenicity concerns. Strict exposure control, suitable protective equipment, and trained laboratory handling are essential.
Product Specifications
| Specification | Details |
|---|---|
| Appearance | White crystalline solid |
| Assay | 99% |
| Chemical Name | Acrylamide |
| Alternate Names | 2-Propenamide, Prop-2-enamide, Acrylic Acid Amide, Propenamide, Acrylamide Monomer |
| Molecular Formula | C3H5NO |
| Empirical Formula | C3H5NO |
| CAS Number | 79-06-1 |
| Molecular Weight | 71.08 g/mol |
| Grade | LR Laboratory Reagent |
Key Features of Acrylamide
- Defined molecular composition: An unsaturated organic amide with the molecular formula C3H5NO and a molecular weight of approximately 71.08 g/mol.
- Polymerization capability: Contains a carbon–carbon double bond that enables polymerization under suitable controlled conditions.
- Water solubility: Dissolves readily in water, supporting preparation of aqueous solutions for appropriate laboratory procedures.
- Electrophoresis research relevance: Used as a monomer for preparing polyacrylamide matrices in established biochemical separation techniques.
- Specialized laboratory handling: Requires stringent exposure controls because the monomer can be absorbed through the skin and presents significant chronic health hazards.
Laboratory Applications of Acrylamide
- Polyacrylamide gel preparation: Used as a monomer in preparing polyacrylamide gels for electrophoresis, following validated laboratory protocols and suitable exposure controls.
- Protein separation research: Supports preparation of gel matrices for sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and related protein separation techniques.
- Molecular biology investigations: Used in research workflows requiring polyacrylamide gels for separating proteins or nucleic acid fragments, depending on the analytical method.
- Polymer chemistry studies: Relevant to controlled investigations of vinyl polymerization, polymer formation, and the properties of polyacrylamide materials.
- Research reagent preparation: May be used to prepare specialized laboratory formulations when the method specifies acrylamide monomer and appropriate engineering controls, protective equipment, and waste procedures are in place.
Why Choose Lab Wale to Buy Acrylamide
Lab Wale provides a sourcing option for laboratory chemicals and scientific supplies used by universities, research centres, analytical laboratories, and scientific institutions. When purchasing Acrylamide in a 250 g pack, buyers should confirm the chemical identity, stated assay, reagent grade, and relevant safety information before use. Reviewing product specifications helps researchers determine whether the material meets the requirements of a particular polymerization or electrophoresis procedure. Because acrylamide monomer presents serious health hazards, laboratories should ensure that appropriate handling facilities, exposure controls, and hazardous waste procedures are available before purchase and use.
Frequently Asked Questions About Acrylamide
What is Acrylamide used for in laboratories?
Acrylamide is primarily used as a monomer for producing polyacrylamide gels in biochemical research, including SDS-PAGE for protein separation. It is also used in polymer chemistry investigations. Because the unpolymerized monomer is hazardous, preparation and handling require suitable exposure controls and trained personnel.
What are the molecular formula and molecular weight of Acrylamide?
Acrylamide has the molecular formula C3H5NO and a molecular weight of approximately 71.08 g/mol. Its empirical formula is also C3H5NO because the subscripts cannot be reduced to a simpler whole-number ratio. These values refer to acrylamide monomer, not polyacrylamide.
What is the CAS number of Acrylamide?
The CAS Registry Number for acrylamide monomer is 79-06-1. This identifier helps distinguish acrylamide from polyacrylamide and other related chemicals during procurement, inventory management, and safety documentation review.
Why is acrylamide monomer hazardous in laboratory work?
Acrylamide monomer can be absorbed through the skin and can damage the nervous system. It is also recognised as a carcinogenic hazard. Laboratories should minimise exposure through appropriate containment, suitable protective equipment, safe work practices, and trained handling. Polymerized polyacrylamide is chemically distinct from the monomer.
How should Acrylamide be stored and handled safely?
Store acrylamide in a tightly closed, compatible container under the temperature and light conditions specified in the applicable Safety Data Sheet. Avoid unnecessary heat and incompatible oxidizing agents because acrylamide can polymerize under unsuitable conditions. Prevent skin contact and dust exposure, use suitable containment, and follow institutional procedures for spills and hazardous waste.
Storage Conditions for Acrylamide
Store Acrylamide in a tightly closed, compatible, clearly labelled container in a cool, dry, well-ventilated location under the conditions specified in the applicable Safety Data Sheet. Protect it from excessive heat and unsuitable light exposure, and keep it away from incompatible oxidizing agents and other reactive substances. Maintain appropriate hazardous chemical storage controls and prevent accidental exposure or release.
Precautions for Handling Acrylamide
Review the current Safety Data Sheet and complete a chemical risk assessment before handling Acrylamide. Use suitable engineering controls, such as an appropriate chemical fume hood or other validated containment, and wear compatible chemical-resistant gloves, protective clothing, and eye protection. Prevent skin contact, inhalation, and dust generation. Restrict handling to trained personnel, avoid uncontrolled heating or polymerization, and follow institutional procedures for spills, decontamination, and hazardous waste disposal.


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