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Acrylamide is an organic compound belonging to the unsaturated amide family, characterized by a reactive carbon–carbon double bond and an amide functional group. Its molecular formula is C3H5NO, and its molecular weight is approximately 71.08 g/mol. This compound is commonly encountered as a white crystalline solid and is readily soluble in water. Acrylamide is an important laboratory reagent in polymer chemistry and biochemical research because it can polymerize to form polyacrylamide, which is widely used in electrophoresis gel preparation and molecular separation techniques. It is also relevant to controlled polymerization studies and investigations of polymer formation. This 500 g pack is intended for appropriate laboratory and research applications where the stated specifications meet the experimental requirements. Acrylamide monomer presents serious health hazards, including neurotoxicity and carcinogenicity concerns, and can be absorbed through the skin. Strict exposure controls, suitable protective equipment, and trained handling are essential.
Product Specifications
| Specification | Details |
|---|---|
| Appearance | White crystalline solid |
| Assay | 99% |
| Chemical Name | Acrylamide |
| Alternate Names | 2-Propenamide, Prop-2-enamide, Acrylic Acid Amide, Propenamide, Acrylamide Monomer |
| Molecular Formula | C3H5NO |
| Empirical Formula | C3H5NO |
| CAS Number | 79-06-1 |
| Molecular Weight | 71.08 g/mol |
| Grade | LR Laboratory Reagent |
Key Features of Acrylamide
- Defined chemical composition: An unsaturated organic amide with the molecular formula C3H5NO and a molecular weight of approximately 71.08 g/mol.
- Polymerization capability: Contains a reactive carbon–carbon double bond that enables polymerization under suitable controlled conditions.
- Water solubility: Dissolves readily in water, supporting the preparation of aqueous solutions for appropriate laboratory procedures.
- Biochemical research relevance: Serves as a monomer for preparing polyacrylamide gel matrices used in established electrophoresis techniques.
- Specialized handling requirements: Requires stringent exposure controls because the unpolymerized monomer can be absorbed through the skin and presents significant health hazards.
Laboratory Applications of Acrylamide
- Polyacrylamide gel preparation: Used as a monomer in preparing gel matrices for electrophoresis, following validated laboratory protocols and appropriate exposure controls.
- Protein separation: Supports preparation of polyacrylamide gels for sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and related protein separation methods.
- Molecular biology research: Used in workflows requiring polyacrylamide matrices for separating proteins or nucleic acid fragments, depending on the analytical method.
- Polymer chemistry investigations: Relevant to controlled studies of vinyl polymerization, polymer formation, and the properties of polyacrylamide materials.
- Research reagent preparation: May be used in specialized laboratory formulations when acrylamide monomer is specified and appropriate containment, protective equipment, and waste procedures are available.
Why Choose Lab Wale to Buy Acrylamide
Lab Wale provides a sourcing option for laboratory chemicals and scientific supplies used by universities, research centres, analytical laboratories, and scientific institutions. When purchasing Acrylamide in a 500 g pack, buyers should review the chemical identity, stated assay, reagent grade, and applicable safety information to determine suitability for the intended procedure. Clear product specifications help researchers select materials for polymerization studies and electrophoresis-related work. Because acrylamide monomer presents serious health hazards, laboratories should ensure that appropriate containment, exposure controls, and hazardous waste procedures are available before use.
Frequently Asked Questions About Acrylamide
What is Acrylamide used for in laboratories?
Acrylamide is primarily used as a monomer for preparing polyacrylamide gels in biochemical research, including SDS-PAGE for protein separation. It is also used in polymer chemistry investigations. Because unpolymerized acrylamide is hazardous, its preparation and handling require suitable exposure controls and trained personnel.
What are the molecular formula and molecular weight of Acrylamide?
Acrylamide has the molecular formula C3H5NO and a molecular weight of approximately 71.08 g/mol. Its empirical formula is also C3H5NO because the subscripts cannot be reduced to a simpler whole-number ratio. These values refer to acrylamide monomer, not polyacrylamide.
What is the CAS number of Acrylamide?
The CAS Registry Number for acrylamide monomer is 79-06-1. This identifier helps distinguish acrylamide from polyacrylamide and other related chemicals during procurement, chemical inventory management, and safety documentation review.
Why is acrylamide monomer hazardous in laboratory work?
Acrylamide monomer can be absorbed through the skin and can damage the nervous system. It is also recognized as a carcinogenic hazard. Laboratories should minimise exposure through appropriate containment, suitable protective equipment, safe work practices, and trained handling. Polymerized polyacrylamide is chemically distinct from the unpolymerized monomer.
How should Acrylamide be stored and handled safely?
Store acrylamide in a tightly closed, compatible container under the temperature and environmental conditions specified in the applicable Safety Data Sheet. Avoid unsuitable heat exposure and incompatible reactive substances. Prevent skin contact and dust exposure, use suitable containment, and follow institutional procedures for spills and hazardous waste disposal.
Storage Conditions for Acrylamide
Store Acrylamide in a tightly closed, compatible, clearly labelled container in a cool, dry, well-ventilated location under the conditions specified in the applicable Safety Data Sheet. Protect it from excessive heat and unsuitable light exposure, and keep it away from incompatible oxidizing agents and other reactive substances. Maintain appropriate hazardous chemical storage controls and prevent accidental exposure or release.
Precautions for Handling Acrylamide
Review the current Safety Data Sheet and complete a chemical risk assessment before handling Acrylamide. Use suitable engineering controls, such as an appropriate chemical fume hood or validated containment, and wear compatible chemical-resistant gloves, protective clothing, and eye protection. Prevent skin contact, inhalation, and dust generation. Restrict handling to trained personnel, avoid uncontrolled heating or polymerization, and follow institutional procedures for spills, decontamination, and hazardous waste disposal.


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