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Dextrose Agar is a dehydrated microbiological culture medium used for cultivating a wide variety of microorganisms under appropriate laboratory conditions. It generally contains dextrose (glucose) as an energy source, peptones and nutrient extracts to provide essential nutrients, sodium chloride to support osmotic balance, and agar as a solidifying agent. The exact composition depends on the manufacturer’s formulation. When prepared according to the applicable instructions, this medium provides a solid surface for microbial cultivation, observation, and selected microbiological investigations. It is relevant to microbiology practicals in colleges and universities, research laboratories, and institutional microbiology facilities. Dextrose Agar may also be supplemented for specialised cultivation procedures when the method permits. Lab Wale offers Dextrose Agar in 1 kg packs for suitable educational and research laboratory applications. Use the medium only for purposes supported by its specific formulation and technical specifications.
Product Specifications
| Specification | Details |
|---|---|
| Appearance | Light beige to pale yellow dehydrated powder |
| Assay | 99% |
| Chemical Name | Dextrose Agar (Dehydrated Microbiological Culture Medium) |
| Alternate Names | Dextrose Agar Medium, Glucose Agar Medium |
| Molecular Formula | Not applicable; composition varies by formulation |
| Empirical Formula | Not applicable; composition varies by formulation |
| CAS Number | Not applicable; this is a formulated mixture of multiple ingredients |
| Molecular Weight | Not applicable; this is a formulated mixture |
| Grade | LR Laboratory Reagent |
Key Features of Dextrose Agar
- Nutrient-containing culture medium: Common formulations contain dextrose, peptones, nutrient extracts, sodium chloride, and bacteriological agar to support microbial cultivation.
- Energy source for microorganisms: Dextrose provides a readily available carbohydrate source that supports microbial growth when the organism’s nutritional requirements are met.
- Solid medium format: Agar provides a solid surface for inoculation, colony development, and observation under appropriate incubation conditions.
- Formulation-dependent characteristics: Nutrient composition, final pH, and preparation requirements vary by manufacturer and should be checked before use.
- Educational and research relevance: Suitable formulations can support supervised microbiology practicals, culture-medium studies, and selected laboratory investigations.
Laboratory Applications of Dextrose Agar
- Microbiology teaching practicals: Used in appropriately supervised experiments to demonstrate microbial cultivation, inoculation techniques, and colony observation.
- General microbial cultivation: Suitable formulations support the cultivation of a range of microorganisms, depending on their nutritional requirements and the medium’s intended use.
- Research laboratory investigations: Used in selected microbiological studies involving growth behaviour and culture-medium performance under controlled conditions.
- Culture-medium preparation studies: Supports practical training in dehydrated medium reconstitution, sterilisation, aseptic dispensing, and preparation of solid culture plates according to the product instructions.
- Supplemented medium preparation: Certain formulations can serve as a base for enriched media when the validated procedure permits appropriate supplementation. The medium’s glucose content may affect specialised observations, including haemolysis assessment.
Why Choose Lab Wale to Buy Dextrose Agar
Lab Wale provides a convenient sourcing option for laboratory chemicals, microbiological media, and scientific supplies for educational institutions, universities, research centres, and laboratory professionals. When purchasing Dextrose Agar, confirm the formulation, intended microbiological application, preparation instructions, and required grade. Reviewing available product specifications and relevant safety information helps laboratories select a suitable medium for their procedures. The 1 kg pack format can support institutional laboratory requirements, subject to the product’s stated specifications and availability.
Frequently Asked Questions About Dextrose Agar
What is Dextrose Agar used for in microbiology laboratories?
Dextrose Agar is used to cultivate a variety of microorganisms under suitable conditions. It provides a carbohydrate source, nutrients, and a solidifying agent for microbial growth. The organisms supported depend on the formulation, incubation conditions, and specific nutritional requirements of the cultures being investigated.
What are the main ingredients of Dextrose Agar?
Common formulations contain dextrose, peptones or protein digests, nutrient extracts, sodium chloride, and bacteriological agar. Dextrose supplies a carbohydrate energy source, nutrient ingredients provide nitrogen and other growth factors, and agar solidifies the medium. Exact ingredient proportions vary by manufacturer.
Does Dextrose Agar have a single molecular formula or CAS number?
No. Dextrose Agar is a formulated mixture rather than a single chemical compound. Its overall composition can vary according to the formulation, so it does not have one universal molecular formula, empirical formula, molecular weight, or CAS Registry Number. Individual ingredients have their own chemical identifiers.
How is dehydrated Dextrose Agar prepared for laboratory use?
Preparation depends on the manufacturer’s instructions. A common formulation is suspended in purified water, heated until dissolved, and sterilised using the specified validated procedure. The prepared medium is then handled aseptically and dispensed as required. Follow the product-specific instructions rather than assuming every formulation has the same preparation requirements.
How should Dextrose Agar be stored and handled safely?
Store the dehydrated powder in its original, tightly closed container in a dry location, protected from moisture and contamination. Wear suitable laboratory clothing, gloves, and eye protection, and minimise dust generation. Handle inoculated cultures using appropriate containment and aseptic practices, and decontaminate used media and cultures according to institutional biosafety procedures.
Storage Conditions for Dextrose Agar
Store dehydrated Dextrose Agar in its original, tightly sealed container in a cool, dry location protected from moisture, excessive heat, and contamination. Many commercial formulations are stored at controlled room temperature, but the exact temperature range should follow the product-specific instructions. Keep the container properly labelled and closed after dispensing to help preserve the medium’s intended performance.
Precautions for Handling Dextrose Agar
Review the applicable Safety Data Sheet and preparation instructions before use. Wear a laboratory coat, suitable gloves, and safety goggles, and avoid inhaling airborne powder during weighing or transfer. Prepare and sterilise the medium using the specified procedure and appropriate equipment. Handle microbial cultures with suitable biosafety precautions, and decontaminate used media and contaminated materials through validated institutional procedures before disposal.


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