Brain Heart Infusion Broth – 500 gram Packs

Brain Heart Infusion Broth (BHI Broth) is a nutrient-rich dehydrated microbiological culture medium designed to support the cultivation of a wide range of microorganisms, including fastidious bacteria that require enriched nutritional conditions. Its typical formulation contains brain and heart infusion solids, peptone, glucose, sodium chloride, and disodium hydrogen phosphate. These ingredients provide amino acids, peptides, vitamins, energy sources, and essential growth factors while supporting osmotic balance and pH stability. Brain Heart Infusion Broth is widely used in microbiology laboratories for bacterial cultivation, enrichment procedures, culture maintenance, and selected investigations involving yeast and fungi when the method is appropriate. The medium is prepared by dissolving the specified quantity in purified water and sterilizing according to validated instructions. This 500 g pack is suitable for laboratory stock and repeated preparation in educational institutions and research facilities. Appropriate microbiological safety practices are essential when handling cultures and prepared media.

Product Specifications

Specification	Details
Appearance	Light beige homogeneous powder
Assay	99%
Chemical Name	Brain Heart Infusion Broth (Dehydrated Microbiological Culture Medium)
Alternate Names	BHI Broth, Brain Heart Infusion Medium, Brain Heart Infusion Liquid Medium Base
Molecular Formula	Not applicable; formulated mixture of multiple ingredients
Empirical Formula	Not applicable; formulated mixture of multiple ingredients
CAS Number	Not applicable; no single CAS number represents the complete mixture
Molecular Weight	Not applicable; formulated mixture of multiple ingredients
Grade	LR Laboratory Reagent

Key Features of Brain Heart Infusion Broth

• Rich nutritional formulation designed to support a broad range of fastidious and non-fastidious microorganisms.
• Contains infusion solids, peptone, glucose, sodium chloride, and phosphate buffer components in a formulation that varies by manufacturer.
• Supports microbial growth through the supply of peptides, amino acids, energy sources, and other nutritional factors.
• Can be used as an enrichment broth or as a base for selected supplemented culture media when permitted by the relevant method.
• Supplied in a 500 g pack for repeated preparation of culture medium in microbiology laboratories.

Laboratory Applications of Brain Heart Infusion Broth

• Cultivation of fastidious bacteria, including suitable species of Streptococcus and Neisseria, under organism-specific laboratory conditions.
• Enrichment of microorganisms in microbiological investigations when the selected analytical method specifies Brain Heart Infusion Broth.
• Educational microbiology practicals demonstrating bacterial growth, nutritional requirements, and the effect of incubation conditions under appropriate supervision.
• Preparation of supplemented culture media, including blood-enriched formulations, when the intended application and validated method support their use.
• Research investigations involving microbial cultivation, growth assessment, and selected studies of yeast or fungi where the medium is suitable for the target organism.

Why Choose Lab Wale to Buy Brain Heart Infusion Broth

Lab Wale offers a convenient sourcing option for laboratory chemicals, microbiological media, and scientific supplies used by schools, colleges, universities, research centres, and laboratory professionals. When purchasing Brain Heart Infusion Broth, review the medium formulation, intended microbiological application, grade, pack size, preparation requirements, and relevant safety information. Selecting a culture medium that matches the target organism and validated testing method helps laboratories plan procurement and maintain consistent procedures. Follow the applicable product instructions and institutional biosafety requirements.

Frequently Asked Questions About Brain Heart Infusion Broth

What is Brain Heart Infusion Broth used for?

Brain Heart Infusion Broth is a nutrient-rich liquid medium used to cultivate a broad range of microorganisms, including many fastidious bacteria. Depending on the formulation and validated method, it may also be used for enrichment, culture maintenance, and selected investigations involving yeast and fungi.

What are the main ingredients in Brain Heart Infusion Broth?

A common formulation contains brain and heart infusion solids, peptone or enzymatic tissue digest, glucose, sodium chloride, and disodium hydrogen phosphate. The infusion components and peptones provide nutrients, glucose supplies an energy source, sodium chloride supports osmotic balance, and phosphate contributes buffering capacity. Exact composition varies by manufacturer.

How is Brain Heart Infusion Broth prepared?

A commonly specified preparation uses approximately 37 g of dehydrated medium per litre of purified water. The powder is dissolved with heating and agitation as directed, dispensed into suitable containers, and sterilized using the validated procedure, often at 121°C for 15 minutes. Always follow the specific product instructions.

Can Brain Heart Infusion Broth support fastidious bacteria?

Yes. Its enriched nutrient composition supports many fastidious organisms, including suitable species of streptococci and other nutritionally demanding bacteria. Growth depends on the organism, medium formulation, atmosphere, temperature, and incubation period. Use organism-specific validated procedures and appropriate biosafety precautions when handling potentially pathogenic cultures.

How should Brain Heart Infusion Broth be stored and handled?

Store the dehydrated medium in a tightly closed container in a cool, dry location within the manufacturer’s specified temperature range. Protect it from moisture and contamination. Wear suitable gloves, protective clothing, and eye protection when preparing the medium, and follow approved procedures for sterilization, handling inoculated cultures, and disposing of microbiological waste.

Storage Conditions for Brain Heart Infusion Broth

Store the dehydrated medium in its original, tightly sealed container in a cool, dry location protected from moisture, direct sunlight, and excessive heat. Follow the manufacturer’s recommended storage temperature and shelf-life instructions. Close the container promptly after dispensing to prevent moisture absorption and contamination. Store prepared broth according to the applicable method and product instructions, and refrigerate it only when the specified procedure requires it.

Precautions for Handling Brain Heart Infusion Broth

Review the applicable Safety Data Sheet and preparation instructions before use. Wear suitable laboratory gloves, protective clothing, and safety goggles, and avoid inhaling powder during weighing and transfer. Use appropriate aseptic techniques when preparing or dispensing sterile medium. Treat inoculated cultures as potentially infectious, apply suitable containment and biosafety procedures, and sterilize contaminated materials before disposal in accordance with institutional requirements and applicable regulations.