Boric Acid Broth- 1 Kg Packs

Boric Acid Broth is a dehydrated microbiological culture medium formulated for the detection and presumptive identification of Escherichia coli based on its ability to grow and produce gas from lactose in the presence of boric acid under specified incubation conditions. The medium contains proteose peptone, lactose, dipotassium phosphate, monopotassium phosphate, and boric acid, which collectively support microbial growth, carbohydrate fermentation, and pH buffering. It is used in microbiology laboratories for educational practicals, water-quality investigations, and research involving the presumptive detection of coliform organisms. When prepared according to an established method, the medium is incubated at an elevated temperature, commonly 43°C, to assess characteristic growth and gas production. This 1 kg pack is suitable for laboratory stock and repeated preparation of culture medium. Use should be restricted to appropriately equipped laboratories following validated microbiological procedures and institutional biosafety requirements.

Product Specifications

Specification	Details
Appearance	Cream to pink homogeneous free-flowing powder
Assay	99%
Chemical Name	Boric Acid Broth (Dehydrated Microbiological Culture Medium)
Alternate Names	Boric Acid Lactose Broth, Boric Acid Broth Medium
Molecular Formula	Not applicable; formulated mixture of multiple ingredients
Empirical Formula	Not applicable; formulated mixture of multiple ingredients
CAS Number	Not applicable; no single CAS number represents the complete mixture
Molecular Weight	Not applicable; formulated mixture of multiple ingredients
Grade	LR Laboratory Reagent

Key Features of Boric Acid Broth

• Specialized dehydrated culture medium formulated for the presumptive detection of Escherichia coli under specified incubation conditions.
• Contains proteose peptone, lactose, phosphate buffers, and boric acid to support the medium’s intended microbiological function.
• Supports assessment of lactose fermentation and gas production using appropriate culture tubes fitted with inverted Durham tubes.
• Has a specified final pH of approximately 7.0 ± 0.2 when prepared at the recommended concentration, subject to the manufacturer’s formulation.
• Supplied in a 1 kg pack for microbiology laboratories requiring repeated preparation of culture medium.

Laboratory Applications of Boric Acid Broth

• Presumptive detection of Escherichia coli based on characteristic growth and gas production at an elevated incubation temperature using an established method.
• Water-quality investigations involving microbiological examination and presumptive assessment of faecal indicator organisms.
• Educational microbiology practicals demonstrating selective growth responses, lactose fermentation, and gas production under supervised laboratory conditions.
• Comparative microbiological testing alongside suitable complementary media to assess characteristic growth responses of suspected coliform organisms.
• Research-centre investigations involving microbiological method development or evaluation, provided the medium is appropriate for the validated protocol and target organism.

Why Choose Lab Wale to Buy Boric Acid Broth

Lab Wale provides a sourcing option for laboratory chemicals, microbiological media, and scientific supplies for educational institutions, research centres, and laboratory professionals. When purchasing Boric Acid Broth, review the product identity, intended microbiological application, grade, pack size, preparation requirements, and relevant safety information. Selecting a medium that matches the target organism and validated test method helps laboratories plan procurement and maintain consistent laboratory procedures. Follow the manufacturer’s preparation instructions and applicable institutional biosafety requirements.

Frequently Asked Questions About Boric Acid Broth

What is Boric Acid Broth used for in microbiology laboratories?

Boric Acid Broth is used for the presumptive detection and identification of Escherichia coli based on characteristic growth and gas production from lactose in the presence of boric acid. It is used in appropriate microbiological investigations involving water samples and other validated testing applications.

What are the main ingredients in Boric Acid Broth?

A commonly specified formulation contains proteose peptone at 10.0 g/L, lactose at 5.0 g/L, dipotassium phosphate at 12.2 g/L, monopotassium phosphate at 4.1 g/L, and boric acid at 3.25 g/L. Exact composition may vary by manufacturer, so follow the formulation supplied for the product.

How is Boric Acid Broth prepared for laboratory use?

A commonly specified preparation method uses approximately 34.55 g of dehydrated medium per litre of distilled or purified water. The prepared medium is dispensed into suitable tubes and sterilized according to the validated method, commonly by autoclaving at 121°C for 15 minutes. Confirm the manufacturer’s instructions before preparation.

What incubation conditions are used with Boric Acid Broth?

A commonly specified method evaluates characteristic growth and gas production after incubation at approximately 43°C for 18–24 hours. Interpretation depends on the target organism, sample preparation, controls, and validated testing procedure. Results are presumptive and should not be treated as definitive identification without appropriate confirmatory testing.

How should Boric Acid Broth be stored and handled safely?

Keep the dehydrated medium tightly closed in a dry, well-ventilated location at the temperature specified by the manufacturer, commonly 10–30°C. Avoid moisture uptake and dust inhalation. Wear appropriate gloves, protective clothing, and eye protection, and consult the applicable Safety Data Sheet for additional precautions related to boric acid and other ingredients.

Storage Conditions for Boric Acid Broth

Store the dehydrated medium in its original, tightly sealed container in a dry, well-ventilated area, protected from moisture, direct sunlight, and temperature extremes. Follow the manufacturer’s specified storage range, commonly 10–30°C, and close the container promptly after dispensing to prevent moisture absorption and lump formation. Store prepared medium according to the applicable method and manufacturer instructions, and observe the stated shelf life.

Precautions for Handling Boric Acid Broth

Review the applicable Safety Data Sheet before handling and wear suitable protective gloves, safety goggles, and laboratory clothing. Avoid inhaling dust and prevent contact with the eyes and skin. Handle the dehydrated medium carefully, using appropriate ventilation where necessary. Prepared cultures and materials exposed to microorganisms must be handled using suitable biosafety practices and decontaminated before disposal according to institutional procedures. Because boric acid may present reproductive hazards, follow the product-specific risk assessment and applicable exposure-control requirements, particularly in educational laboratories.