B.C.G. Dextrose Agar- 1 Kg Packs

B.C.G. Dextrose Agar, also known as Bromocresol Green Dextrose Agar or Snyder Test Agar, is a specialized dehydrated culture medium used in microbiology to estimate Lactobacilli and assess microbial acid production associated with dental caries activity. It contains peptic digest of animal tissue, dextrose, sodium chloride, bromocresol green, and agar to support microbial growth and indicate acid formation. When dextrose-fermenting microorganisms produce acid, the pH decreases and the bromocresol green indicator changes from green toward yellow. This visible colour response helps laboratories evaluate the rate and extent of acid production under controlled test conditions. B.C.G. Dextrose Agar is relevant to microbiology teaching, dental research, and supervised laboratory investigations involving oral microorganisms. Its performance depends on correct preparation, incubation conditions, and appropriate microbiological practices. It is intended for laboratory use by trained personnel following the applicable product instructions.

Product Specifications

Specification	Details
Appearance	Light yellow to greenish-yellow homogeneous free-flowing powder
Assay	99%
Chemical Name	B.C.G. Dextrose Agar (Snyder Test Agar)
Alternate Names	Bromocresol Green Dextrose Agar, Snyder Test Agar
Molecular Formula	Not applicable; formulated culture medium mixture
Empirical Formula	Not applicable; formulated culture medium mixture
CAS Number	Not applicable; formulated culture medium mixture
Molecular Weight	Not applicable; formulated culture medium mixture
Grade	LR Laboratory Reagent

Key Features of B.C.G. Dextrose Agar

• Specialized microbiological medium: Formulated for estimating Lactobacilli and evaluating acid production associated with dental caries activity.
• Dextrose-based fermentation system: Dextrose provides a fermentable carbohydrate that supports the assessment of microbial acid production.
• pH indicator response: Bromocresol green provides a visible colour change from green toward yellow as acidic products lower the medium’s pH.
• Solid culture format: Agar provides a gel matrix suitable for preparing culture medium in tubes under the prescribed laboratory conditions.
• Defined working pH: The standard formulation has a final pH of approximately 4.8 ± 0.2 at 25°C, supporting the intended differential test principle.

Laboratory Applications of B.C.G. Dextrose Agar

• Lactobacilli estimation: Used in Snyder test procedures to estimate Lactobacilli associated with oral microbial activity.
• Dental caries activity research: Supports controlled investigations of acid production by oral microorganisms as an indicator associated with caries activity.
• Microbial carbohydrate fermentation studies: Helps demonstrate how dextrose fermentation can produce acidic metabolites and change the pH of a culture medium.
• Educational microbiology practicals: Suitable for supervised college and university teaching activities involving culture media, microbial metabolism, and pH indicators, subject to institutional biosafety requirements.
• Dental microbiology investigations: May support research involving acidogenic oral microorganisms when the procedure, interpretation, and limitations of the method are appropriately established.

Why Choose Lab Wale to Buy B.C.G. Dextrose Agar

Lab Wale provides a sourcing option for laboratory chemicals, microbiological culture media, and scientific supplies used by educational institutions, universities, research centres, and laboratory professionals. When purchasing B.C.G. Dextrose Agar, laboratories should verify the product identity, formulation, stated grade, preparation instructions, and applicable technical and safety information. Selecting a medium designed for the intended microbiological test helps support appropriate laboratory planning and consistent procedures. Proper storage, preparation, quality control, and microbiological safety practices remain essential for reliable laboratory work.

Frequently Asked Questions About B.C.G. Dextrose Agar

What is B.C.G. Dextrose Agar used for?

B.C.G. Dextrose Agar, or Snyder Test Agar, is used to estimate Lactobacilli and assess acid production associated with dental caries activity. The medium contains dextrose and a pH indicator that responds to acid formation. Results must be interpreted according to the prescribed test method rather than as a standalone clinical diagnosis.

What is the principle of Snyder Test Agar?

The principle is based on acid production during microbial fermentation of dextrose. As microorganisms produce acidic metabolites, the pH decreases and bromocresol green changes from green toward yellow. The rate and extent of this colour change provide an indication of acid production under the specified test conditions.

What is the composition and pH of B.C.G. Dextrose Agar?

A common formulation contains peptic digest of animal tissue, dextrose, sodium chloride, bromocresol green, and agar. The standard final pH is approximately 4.8 ± 0.2 at 25°C. Exact ingredient specifications and preparation quantities should be checked against the technical information for the particular product.

How is B.C.G. Dextrose Agar prepared for laboratory use?

Follow the manufacturer’s instructions for the specific formulation. A common preparation uses approximately 65 g of dehydrated medium per litre of distilled or purified water, with heating to dissolve and sterilization by autoclaving at 121°C for 15 minutes. Avoid overheating and follow the specified dispensing, incubation, and quality-control procedures.

How should B.C.G. Dextrose Agar be stored and handled?

Store the dehydrated medium in a tightly sealed container in a cool, dry place, protected from moisture and direct sunlight. Wear suitable laboratory protective equipment and follow good microbiological practices. Treat inoculated cultures and used media as potentially biohazardous, and sterilize or dispose of them according to institutional biosafety procedures.

Storage Conditions for B.C.G. Dextrose Agar

Store the dehydrated B.C.G. Dextrose Agar in its tightly closed, moisture-resistant container in a cool, dry area, protected from direct sunlight and contamination. Follow the product-specific storage temperature stated by the manufacturer; some formulations specify storage below 30°C. Keep the container securely closed after use and avoid exposure to humidity, which can affect the quality of the medium.

Precautions for Handling B.C.G. Dextrose Agar

Review the product’s current safety information before use and wear a laboratory coat, suitable gloves, and eye protection. Avoid inhaling airborne powder and prevent contact with the eyes. Prepare the medium using appropriate laboratory equipment and follow the prescribed sterilization procedure. Handle inoculated media and clinical specimens using established microbiological safety practices, and sterilize or dispose of used cultures in accordance with institutional biosafety and waste-management requirements.