Eosine Yellow Indicator for Microscopy Water Soluble- 25gm Packs

Eosine Yellow Indicator for Microscopy, Water Soluble, is a synthetic acidic dye used in histology, biological staining, and microscopic examination. Commonly known as Eosin Y, the water-soluble disodium salt is used to impart pink to red coloration to compatible tissue components, helping improve contrast during microscopic observation. It is widely used as a counterstain in hematoxylin and eosin (H&E) staining, where it helps distinguish cytoplasm and selected extracellular structures from blue-purple stained cell nuclei. Its water-soluble form is suitable for preparing aqueous staining solutions according to established laboratory procedures. Eosine Yellow is relevant to educational microscopy practicals, histology laboratories, pathology research, and biological investigations involving prepared tissue specimens. Staining performance depends on dye concentration, pH, staining time, fixation, and the specimen preparation method. Laboratories should confirm the exact dye identity and product specifications before use and follow appropriate chemical handling and waste disposal procedures.

Product Specifications

Key Features of Eosine Yellow Indicator for Microscopy Water Soluble

• Water-soluble synthetic acidic dye suitable for preparing aqueous staining solutions.
• Produces pink to red coloration in compatible eosinophilic tissue components.
• Commonly used as a counterstain in hematoxylin and eosin staining procedures.
• Helps improve contrast between cell nuclei and surrounding tissue structures during microscopy.
• Useful for educational histology practicals and suitable biological research procedures, subject to method compatibility.

Laboratory Applications of Eosine Yellow Indicator for Microscopy Water Soluble

• Histological staining: Used as an eosin counterstain in H&E procedures to improve contrast between nuclei and other tissue components.
• Educational microscopy: Supports supervised laboratory practicals involving tissue staining, microscope slide preparation, and specimen observation.
• Pathology research: Used in compatible tissue-staining workflows to assist morphological examination and documentation.
• Tissue component visualisation: Helps demonstrate the staining characteristics of cytoplasm and selected extracellular structures in appropriately prepared specimens.
• Aqueous stain preparation: Used to prepare water-based staining solutions when specified by the laboratory protocol, with concentration and pH controlled as required.

Why Choose Lab Wale to Buy Eosine Yellow Indicator for Microscopy Water Soluble

Lab Wale provides a sourcing option for laboratory chemicals, microscopy stains, and scientific supplies used by schools, colleges, universities, research centres, and laboratory professionals. When purchasing Eosine Yellow Water Soluble, laboratories should confirm the chemical identity, water-soluble form, grade, and compatibility with their staining procedures. Reviewing available technical and safety documentation helps users select an appropriate reagent for their intended application. Lab Wale supports laboratory purchasing requirements for educational, histological, and biological research applications.

Frequently Asked Questions About Eosine Yellow Indicator for Microscopy Water Soluble

What is Eosine Yellow Water Soluble used for in microscopy?

Eosine Yellow Water Soluble is an acidic biological dye used to stain compatible tissue components pink to red. It is commonly used as a counterstain in hematoxylin and eosin staining and helps distinguish cytoplasm and selected extracellular structures during microscopic examination of prepared specimens.

What is the molecular formula of Eosine Yellow Water Soluble?

The molecular formula of Eosin Y disodium salt is C₂₀H₆Br₄Na₂O₅, and its molecular weight is approximately 691.88 g/mol. Its empirical formula is identical because the subscripts cannot be reduced to a smaller whole-number ratio.

What is the CAS number of Eosine Yellow Water Soluble?

CAS number 17372-87-1 is associated with Eosin Y disodium salt. The dye is also known as Acid Red 87 and is identified by Colour Index number 45380. Laboratories should confirm the precise chemical form against the product documentation before selecting a reagent for a particular staining procedure.

Why is Eosine Yellow used with hematoxylin in histology?

Hematoxylin typically stains cell nuclei blue to purple, whereas eosin stains many cytoplasmic and extracellular components pink to red. This colour contrast helps distinguish tissue structures during microscopic examination. The final staining pattern depends on specimen preparation, fixation, reagent concentration, pH, and the staining protocol.

How should Eosine Yellow Water Soluble be stored and handled?

Store the powder in a tightly closed container in a cool, dry place, protected from moisture and contamination. Wear suitable gloves, laboratory clothing, and eye protection, and avoid inhaling dust. Consult the current Safety Data Sheet for product-specific hazards, ventilation requirements, spill response, and disposal instructions.

Storage Conditions for Eosine Yellow Indicator for Microscopy Water Soluble

Store Eosine Yellow Water Soluble in a compatible, tightly closed, clearly labelled container in a cool, dry area protected from moisture, excessive light, and contamination. Close the container promptly after use to minimise exposure to ambient humidity. Follow the product-specific Safety Data Sheet for any additional temperature limits or storage requirements.

Precautions for Handling Eosine Yellow Indicator for Microscopy Water Soluble

Review the current Safety Data Sheet before use and wear suitable laboratory gloves, safety goggles, and protective clothing. Avoid generating or inhaling powder dust and prevent unnecessary contact with skin and eyes. Use appropriate ventilation when weighing the dye or preparing solutions. Clean spills using approved laboratory procedures and dispose of dye solutions and contaminated materials according to institutional requirements and applicable regulations.