Eosine Yellow Indicator for Microscopy Water Soluble- 100gm Packs

Eosine Yellow Indicator for Microscopy, Water Soluble, is a synthetic acidic dye used in histology, biological staining, and microscopic examination. Commonly identified as Eosin Y disodium salt, also known as Acid Red 87, this brominated fluorescein dye binds preferentially to suitable positively charged components of biological specimens, producing pink to red staining. It is widely used as a counterstain in hematoxylin and eosin (H&E) staining, helping distinguish cytoplasm, connective tissue components, and other eosinophilic structures from darkly stained cell nuclei. Its water-soluble form is suitable for preparing aqueous staining solutions according to established laboratory protocols. Eosine Yellow is valuable in educational microscopy practicals, histology laboratories, pathology research, and biological investigations requiring clear tissue contrast. The dye’s performance depends on solution preparation, pH, staining time, and the specimen preparation method. Appropriate handling precautions should be followed to minimise exposure to the powder and staining solutions.

Product Specifications

Key Features of Eosine Yellow Indicator for Microscopy Water Soluble

• Water-soluble synthetic acidic dye suitable for preparing aqueous microscopic staining solutions.
• Provides pink to red staining of compatible eosinophilic tissue components.
• Commonly used as a counterstain alongside hematoxylin in histological staining procedures.
• Supports differentiation of cytoplasmic and extracellular tissue structures during microscopic observation.
• Useful in educational, histology, and biological research laboratories following validated staining protocols.

Laboratory Applications of Eosine Yellow Indicator for Microscopy Water Soluble

• Histological staining: Used as an eosin counterstain in H&E procedures to improve contrast between tissue components and cell nuclei.
• Microscopy practicals: Supports supervised educational exercises involving tissue staining, slide preparation, and microscopic identification.
• Pathology research: Used in compatible tissue-staining workflows to support morphological examination and documentation.
• Cell and tissue differentiation: Helps visualise eosinophilic structures, including cytoplasm and selected extracellular components, in appropriately prepared specimens.
• Staining solution preparation: Used to prepare aqueous dye solutions for laboratory protocols that specify water-soluble Eosin Y, with concentration and pH adjusted as required.

Why Choose Lab Wale to Buy Eosine Yellow Indicator for Microscopy Water Soluble

Lab Wale provides a sourcing option for laboratory chemicals, microscopy stains, and scientific supplies used by schools, colleges, universities, research centres, and laboratory professionals. When purchasing Eosine Yellow Water Soluble, laboratories should check the chemical identity, water solubility, grade information, and suitability for the intended staining procedure. Available technical and safety documentation can help users select the appropriate reagent and establish safe handling practices. Lab Wale supports laboratory purchasing requirements for educational, histological, and biological research applications.

Frequently Asked Questions About Eosine Yellow Indicator for Microscopy Water Soluble

What is Eosine Yellow Water Soluble used for in microscopy?

Eosine Yellow Water Soluble is used as an acidic biological stain to colour compatible tissue components pink to red. It is commonly used as a counterstain in hematoxylin and eosin staining and supports the microscopic examination of cytoplasm and selected extracellular structures in appropriately prepared specimens.

What is the molecular formula of Eosine Yellow Water Soluble?

The molecular formula of Eosine Yellow Disodium Salt is C₂₀H₆Br₄Na₂O₅, with a molecular weight of approximately 691.88 g/mol. Its empirical formula is the same because the subscripts cannot be reduced to a smaller whole-number ratio.

What is the CAS number of Eosine Yellow Water Soluble?

The CAS Registry Number for Eosine Yellow Water Soluble, commonly identified as Eosin Y disodium salt, is 17372-87-1. It is associated with Colour Index number 45380. Confirm the identity against the product documentation when selecting a stain for a specific microscopy procedure.

Why is Eosine Yellow used with hematoxylin in histology?

Hematoxylin typically stains cell nuclei blue to purple, while eosin stains many cytoplasmic and extracellular components pink to red. This contrasting colour pattern helps distinguish tissue structures during microscopic examination. The final staining quality depends on fixation, differentiation, staining conditions, and the preparation protocol.

How should Eosine Yellow Water Soluble be stored and handled?

Store the powder in a tightly closed container, protected from moisture, contamination, and excessive light. Wear suitable gloves, protective clothing, and eye protection, and avoid inhaling dust. Consult the current Safety Data Sheet for product-specific hazards, ventilation requirements, spill management, and disposal procedures.

Storage Conditions for Eosine Yellow Indicator for Microscopy Water Soluble

Store Eosine Yellow Water Soluble in a tightly closed, compatible, clearly labelled container in a cool, dry area protected from moisture, excessive light, and contamination. Avoid unnecessary exposure to heat and airborne humidity. Keep the material in a suitable chemical storage area and follow the product-specific Safety Data Sheet for any additional storage requirements.

Precautions for Handling Eosine Yellow Indicator for Microscopy Water Soluble

Review the current Safety Data Sheet before handling. Wear suitable chemical-resistant gloves, protective clothing, and safety goggles, and avoid generating or inhaling powder dust. Use appropriate ventilation when weighing the dye or preparing solutions, and prevent contact with skin and eyes. Clean spills using approved laboratory procedures and dispose of dye solutions and contaminated materials according to institutional requirements.