Crystal Violet Powder (25gm)

Crystal Violet Powder is a synthetic triphenylmethane cationic dye commonly known as Gentian Violet or Basic Violet 3. Its chemical formula is C₂₅H₃₀ClN₃, and its molecular weight is approximately 407.98 g/mol. The compound is characterised by its intense violet colour and ability to bind to various biological and laboratory materials. Crystal Violet is widely used in microbiology and educational laboratories, particularly as the primary stain in Gram staining procedures for differentiating bacterial cell types. It is also used in microscopy, histological staining, and selected research procedures involving dye binding and colourimetric investigations. Its solubility in water supports the preparation of suitable staining solutions according to established laboratory protocols. This 25 gram pack is intended for laboratory and research applications where the specified chemical identity and reagent grade meet the method requirements. Appropriate protective equipment and safe chemical-handling procedures are essential during use.

Product Specifications

Specification	Details
Appearance	Dark green to violet crystalline powder
Assay	99%
Chemical Name	Crystal Violet
Alternate Names	Gentian Violet, Basic Violet 3, Methylrosanilinium Chloride, C.I. 42555
Molecular Formula	C₂₅H₃₀ClN₃
Empirical Formula	C₂₅H₃₀ClN₃
CAS Number	548-62-9
Molecular Weight	407.98 g/mol
Grade	LR Laboratory Reagent

Key Features of Crystal Violet Powder

• Distinctive violet dye: Crystal Violet is a cationic triphenylmethane dye with strong violet coloration and useful staining characteristics.
• Defined chemical composition: The anhydrous chloride salt has the molecular formula C₂₅H₃₀ClN₃ and a molecular weight of approximately 407.98 g/mol.
• Water solubility: It is soluble in water, allowing preparation of suitable aqueous staining solutions under controlled laboratory conditions.
• Microbiological staining applications: Serves as the primary stain in the Gram staining method used in bacterial differentiation.
• Research laboratory relevance: Supports microscopy, selected histological staining procedures, and investigations involving dye binding and colour measurement.

Laboratory Applications of Crystal Violet Powder

• Gram staining: Used as the primary stain in Gram staining protocols for differentiating Gram-positive and Gram-negative bacteria when the complete validated staining procedure is followed.
• Microbiology practicals: Used in supervised educational laboratory exercises involving bacterial staining, microscopy, and observation of microbial morphology.
• Histological staining: Applied in selected staining procedures for laboratory specimens, depending on the protocol and sample type.
• Cell and material staining research: Used in selected investigations of dye binding to biological materials, surfaces, and laboratory samples.
• Colourimetric investigations: May be used in research procedures that measure dye concentration or changes in absorbance using a suitable analytical method.

Why Choose Lab Wale to Buy Crystal Violet Powder

Lab Wale provides a convenient sourcing option for laboratory chemicals and scientific supplies used by schools, colleges, universities, microbiology laboratories, research centres, and laboratory professionals. When purchasing Crystal Violet Powder, check the chemical identity, stated reagent grade, pack size, and available technical and safety information to ensure suitability for the intended staining or research procedure. The 25 gram pack offers a defined quantity for laboratory requirements. Selecting an appropriate reagent and following validated laboratory protocols helps support consistent staining procedures and responsible chemical handling.

Frequently Asked Questions About Crystal Violet Powder

What is Crystal Violet Powder used for in laboratories?

Crystal Violet is primarily used as a laboratory stain, particularly as the primary dye in Gram staining for bacterial differentiation. It is also used in selected microscopy, histological staining, and dye-binding research procedures. The appropriate formulation and concentration depend on the validated protocol and intended sample.

What is the molecular formula of Crystal Violet?

The molecular formula of anhydrous Crystal Violet chloride is C₂₅H₃₀ClN₃, and its molecular weight is approximately 407.98 g/mol. Some commercial products are supplied as hydrated forms, which have different formulas and molecular weights. Confirm the supplied form when calculating concentrations or preparing laboratory solutions.

What is the CAS number of Crystal Violet Powder?

The CAS Registry Number for anhydrous Crystal Violet chloride is 548-62-9. It is also known as Gentian Violet and Basic Violet 3. Hydrated forms may have different identifiers, so the exact product identity should be checked before using the chemical in analytical or research procedures.

Why is Crystal Violet used in Gram staining?

Crystal Violet acts as the primary stain in the Gram staining procedure. When combined with iodine, it forms a dye complex that is retained more effectively by Gram-positive bacteria during decolourisation. Gram-negative bacteria generally lose the primary stain during this step and are subsequently visualised using a counterstain.

How should Crystal Violet Powder be stored and handled safely?

Store Crystal Violet in a tightly closed container in a cool, dry location, protected from unnecessary light exposure. Avoid inhaling dust and prevent contact with the eyes and skin. Wear suitable gloves, protective eyewear, and laboratory clothing, consult the applicable Safety Data Sheet, and collect waste for approved chemical disposal.

Storage Conditions for Crystal Violet Powder

Store Crystal Violet Powder in a tightly closed, compatible container in a cool, dry, well-ventilated area, protected from excessive heat and unnecessary light exposure. Avoid contamination and the formation of airborne dust. Keep the material secured and segregated from incompatible substances according to the applicable Safety Data Sheet. Maintain clear labelling and follow institutional chemical-storage procedures.

Precautions for Handling Crystal Violet Powder

Review the current Safety Data Sheet before use and wear suitable chemical-resistant gloves, safety goggles, and laboratory clothing. Avoid inhaling dust and prevent contact with the eyes and skin. Use appropriate ventilation when weighing the powder or preparing staining solutions. Crystal Violet has significant health-hazard concerns, including suspected carcinogenicity in relevant safety classifications, so minimise exposure and follow institutional controls. Prevent environmental release and dispose of solutions, contaminated materials, and residues through approved chemical-waste procedures.