Charcoal Agar Base- 1 Kg Packs

Charcoal Agar Base is a dehydrated microbiological culture medium used for the cultivation and isolation of fastidious bacteria, particularly Bordetella pertussis and, in suitable formulations, Haemophilus influenzae. This specialized laboratory medium contains nutritional ingredients such as peptones and beef extract, together with charcoal, starch, sodium chloride, nicotinic acid, and agar, depending on the formulation. The nutrients support microbial growth, while charcoal and starch help neutralize inhibitory substances that may interfere with bacterial recovery. Agar provides the solidifying matrix required for preparing culture plates. Charcoal Agar Base is used in microbiology teaching, culture-based research, and appropriate bacteriological investigations. The 1 kg pack is suitable for laboratories with planned medium preparation requirements. Supplementation, preparation procedures, incubation conditions, and intended applications must follow the specific manufacturer’s instructions and the laboratory’s established microbiological safety protocols.

Product Specifications

Key Features of Charcoal Agar Base

• Specialized microbiological medium: Designed for the cultivation of selected fastidious bacteria, particularly Bordetella species, when used according to the specified formulation.
• Nutrient-rich composition: Contains nutritional ingredients that support bacterial growth, with the precise formulation depending on the manufacturer.
• Charcoal-based inhibitory substance absorption: Charcoal and starch help neutralize certain inhibitory metabolites that can affect the recovery of susceptible microorganisms.
• Solid culture medium preparation: Agar provides the gelling matrix needed to prepare solid microbiological media.
• Convenient institutional pack: The 1 kg pack supports planned medium preparation in microbiology teaching laboratories and research facilities.

Laboratory Applications of Charcoal Agar Base

• Microbiology practicals: Used in appropriately supervised educational laboratories to demonstrate culture media preparation and fundamental bacteriological techniques with approved teaching organisms.
• Cultivation of Bordetella species: Supports the cultivation of suitable Bordetella species, including Bordetella pertussis, when the formulation and preparation method meet the organism’s growth requirements.
• Haemophilus cultivation: Certain formulations support the cultivation of Haemophilus influenzae; suitability depends on the formulation, supplementation, and organism-specific requirements.
• Microbiological research: Used in appropriately designed culture-based investigations involving bacterial growth and the performance of specialized culture media.
• Culture medium evaluation: Applied in laboratory studies assessing medium preparation, growth-supporting characteristics, and consistency under established quality-control procedures.

Why Choose Lab Wale to Buy Charcoal Agar Base

Lab Wale provides a sourcing option for laboratory chemicals, microbiological media, and scientific supplies used by educational institutions, universities, research centres, and laboratory professionals. When purchasing Charcoal Agar Base, laboratories should verify the intended formulation, pack size, preparation instructions, and suitability for their target microorganisms. Reviewing the applicable technical and safety documentation helps ensure that the medium matches the intended laboratory procedure. The 1 kg pack offers a practical purchasing format for institutions planning routine microbiological media preparation and inventory requirements.

Frequently Asked Questions About Charcoal Agar Base

What is Charcoal Agar Base used for in microbiology laboratories?

Charcoal Agar Base is used to prepare solid culture media for cultivating selected fastidious bacteria, particularly Bordetella pertussis. Depending on its formulation, it may also support other organisms. The intended application depends on the medium’s composition, required supplements, and the manufacturer’s instructions.

What are the main ingredients of Charcoal Agar Base?

Common formulations contain beef extract or another nutrient source, peptones, charcoal, starch, sodium chloride, agar, and sometimes nicotinic acid. The exact ingredients and concentrations vary between manufacturers. Always check the formulation supplied with the product before preparing the culture medium.

Does Charcoal Agar Base have a molecular formula or CAS number?

No single molecular formula, empirical formula, molecular weight, or universal CAS number describes Charcoal Agar Base as a whole because it is a mixture of multiple ingredients. Individual components have their own chemical identifiers, but these should not be presented as identifiers for the complete culture medium.

Does Charcoal Agar Base require additional supplements?

Some formulations require additional supplements to support specific organisms or provide selective properties. For example, certain Bordetella culture procedures use blood supplementation, while selective formulations may include an appropriate antimicrobial supplement. Follow the manufacturer’s instructions for the exact product rather than assuming all formulations require identical additions.

How should Charcoal Agar Base be stored and handled?

Store the dehydrated medium in its original, tightly closed container under the temperature conditions specified by the manufacturer. Protect it from moisture and excessive light where required. Avoid inhaling the powder, wear appropriate personal protective equipment, and handle prepared cultures using established microbiological safety and waste-disposal procedures.

Storage Conditions for Charcoal Agar Base

Store Charcoal Agar Base in its original, tightly sealed container in a cool, dry location, following the manufacturer’s specified temperature range. Many dehydrated formulations are stored between 10°C and 30°C, but product-specific instructions take precedence. Protect the powder from moisture and direct light where required, and avoid contamination during use. Store prepared culture media according to the applicable instructions for the specific formulation.

Precautions for Handling Charcoal Agar Base

Review the product’s current Safety Data Sheet and preparation instructions before use. Wear a laboratory coat, suitable gloves, and protective eyewear, and avoid inhaling airborne powder. Prepare and sterilize the medium using validated laboratory procedures and appropriate equipment. Treat inoculated cultures and microbiological waste according to institutional biosafety requirements, especially when potentially pathogenic organisms are involved. Ensure that culture preparation and handling are performed by trained personnel under appropriate laboratory supervision.