25 Differences Between Immunoassays and Chromatography Techniques in Lab Testing

Two crucial methods utilized in laboratory testing, particularly in the domains of analytical chemistry, clinical diagnostics, and biochemistry, are chromatography and immunoassays. These techniques are essential for the detection and examination of a wide range of materials, including chemical compounds and biomolecules.

Biochemical tests known as immunoassays use the interaction between antibodies and antigens to identify and measure a variety of chemicals, including medicines, proteins, hormones, and infectious agents.

The immunological system’s specificity is the basis of immunoassay principles. Target molecules (antigens) are recognised and bound to by antibodies, which are proteins made by the immune system in reaction to particular antigens.

In clinical laboratories, immunoassays are frequently used for disease diagnosis, medication level monitoring, infectious agent detection, and research in a variety of domains.

Chromatography is a technique for chemical separation that is used to analyze and separate mixtures of substances according to how differently they bind to a stationary phase and a mobile phase.

The distribution of components between the mobile phase, which is a liquid or gas that passes through the stationary phase, and the stationary phase, which is a solid or liquid support, is the basis of chromatography. Separation results from the movement of components with varying affinities for various phases at different rates.

Chromatography is used to separate, identify, and measure diverse components in complicated mixtures in a variety of applications, including forensics, environmental studies, food and beverage testing, medicines, and clinical diagnostics.

Both chromatography and immunoassays are flexible methods with many uses in the lab that yield insightful data for investigations, diagnosis, and quality assurance.

Frequently Asked Questions (FAQ’S)