Triple Sugar Iron Agar (500 ml)

Triple Sugar Iron Agar (TSI) in a 500 ml volume is specially formulated for identifying and differentiating Gram-negative enteric bacteria. It detects the fermentation of glucose, lactose, and sucrose, along with hydrogen sulfide production. This medium supports reliable and rapid biochemical testing, helping microbiologists accurately diagnose infections and study bacterial metabolism under controlled laboratory conditions.

Key Features

1. Supports differentiation of enteric bacteria based on sugar fermentation.
2. Detects hydrogen sulfide gas production with iron salts.
3. Clear and reliable color changes for easy interpretation.
4. Prepared according to international microbiological standards.
5. Available in convenient 500 ml volume for multiple tests.

Precautions

1. Store in a cool, dry place away from direct sunlight.
2. Use aseptic techniques to prevent contamination during inoculation.
3. Do not use if the medium appears discolored or has precipitates.
4. Handle with care to avoid spills and exposure to skin.
5. Follow proper disposal guidelines for used media and cultures.

Frequently Asked Questions (FAQs)

Question: What is the primary use of Triple Sugar Iron Agar?
Answer: It is used to differentiate Gram-negative enteric bacteria based on their ability to ferment sugars and produce hydrogen sulfide.

Question: How should the Triple Sugar Iron Agar be stored?
Answer: Store it in a cool, dry place away from direct sunlight to maintain its efficacy.

Question: Can this medium detect hydrogen sulfide production?
Answer: Yes, the medium contains iron salts that react with hydrogen sulfide to form a black precipitate.

Question: Is this product ready to use or needs preparation?
Answer: This is a prepared medium ready for immediate use upon delivery.

Question: How long can the Triple Sugar Iron Agar be stored after opening?
Answer: It is recommended to use the medium soon after opening and within the shelf life indicated on the packaging for best results.